ISE Electrodes

Direct Measurement
A simple procedure for measuring a large number of samples. Each sample only requires one reading. Only a small sample volume is required. Calibration is performed on a series of standards. The concentration is then determined by comparison to the standards. Ionic strength adjustor is added to all solutions to ensure samples and standards have similar ionic strength, proper pH and reduce the effect of interfering ions. Orion ISE meters calculate and store the calibration curves.

Low Level Measurement
A similar method to direct measurement. It is recommended when the sample is not in the linear response range. A minimum 3 point calibration is recommended to compensate for the non-linear response. Calibration is performed in one beaker reducing the chance of cross contamination of the standards.

Known Addition
A useful method for measuring samples since calibration is not required. This method is recommended when measuring only a few samples, when samples have a high ionic strength (>0.1 M) or when there is a complicated background matrix. An aliquot of standard solution containing the measured species is added to the sample. The sample concentration is determined by the changes in potential before and after the addition. Orion ISE meters automatically calculate the result.

Analate Subtraction
A useful method for measuring samples since calibration is not required. The electrodes are immersed in a reagent solution that contains a species that the electrode senses and then it reacts with the sample. It is useful when sample size is small, for samples for which a standard is difficult to prepare, and for viscous or very concentrated samples. The method is not suited for very diluted samples. It is also necessary to know the stoichiometric ration between sample and standard.

Quantitative analytical techniques for measuring the concentration of a species by incremental addition of a reagent (titrant) that reacts with the sample species. Sensing electrodes can be used for determination of the titration end point. Ion selective electrodes are useful as end point detectors because they are unaffected by sample color or turbidity.

Fluoride Ammonia Nitrate Chloride